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Table 2 List of primers used for HPV infection, HPV typing, STAT3 RT PCR and quality control of RNAa

From: Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis

Primers Amplicon size Primer sequence
MY09/MY11 β-Globin 450 bp 268 bp 5’- GCM CAG GGW CAT AAY AAT GG -3’ 5’- CGT CCM ARR GGA WAC TGA -3’ Where M = A or C, W = A or T, Y = C or T and R = A or G 5’- GAA GAG CCA AGG ACA GGT AC-3’ 5’- CAA CTT CAT CCA CGT TAC ACC-3’
HPV16 217 bp 5’- AAG GCC AAC TAA ATG TCA C -3’ 5’- CTG CTT TTA TAC TAA CCG G -3’
HPV18 322 bp 5’- ACC TTA ATG AAA AAC CAC GA -3’ 5’- CGT CGT TTA GAG TGG TTC GTG -3’
STAT3 RT 318 bp 5’-TTGCCAGTTGTGGTGATC-3’ 5’-GAACCCAGAAGGAGAAGC-3’
GAPDH RT 322 bp 5’-TGGATATTGTTGCCATCA ATGACC-3’ 5’-GATGGCATGGACTGTGGTCATG-3’
  1. aList of primers and probes for PGMY reverse line blot-based comprehensive genotyping and the methodology can be obtained through WHO HPV laboratory manual (First Edition; 2010)