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Table 3 List of primers used for microRNA-21 & Let-7a qRT-PCR in the study

From: Alterations in microRNAs miR-21 and let-7a correlate with aberrant STAT3 signaling and downstream effects during cervical carcinogenesis

Primer name Primer sequence
U6 RT 5' CGCGCCTGCAGGTCGACAATTAACCCTCACTAAAGGGttgcgtgtcatcc 3'
U6 PCR (F) 5' GTAATACGACTCACTATAGGGAGAAGAGcctgcgcaagg 3'
U6 PCR (R) 5' CGCGCCTGCAGGTCGAC 3'
miR-21 RT 5' CGCGCCTGCAGGTCGACAATTAACCCTCACTAAAGGGtcaacatcagtct 3'
miR-21 PCR (F) 5' GTAATACGACTCACTATAGGGAGAAGAGtagcttatcag 3'
miR-21 PCR (R) 5' CGCGCCTGCAGGTCGAC 3'
Let-7a RT 5' CGCGCCTGCAGGTCGACAATTAACCCTCACTAAAGGGaactatacaacct 3'
Let-7a PCR (F) 5' GTAATACGACTCACTATAGGGAGAAGAGtgaggtagtag 3'
Let-7a PCR (R) 5' CGCGCCTGCAGGTCGAC 3'
  1. The capital letters indicate universal primers sequence of miRNAs for RT and forward primer of each miR while small letters sequence is specific for the miRNA of interest which binds to the miRNA. The amplicon size for each miR is 90 bp