Fig. 2
From: COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer
Aberrant mRNA expression levels of GalNAc-transferases, T-Synthase and COSMC as well as T-synthase activity in COSMC-depleted pancreatic cancer cells. a Relative mRNA expression of all human GalNAc-transferase isoforms in COSMC knockdown as well as control Panc-1 cells, quantified using real-time PCR. b Relative quantification of T-synthase mRNA expression in COSMC knockdown and control Panc-1 cells. Note: mRNA expression level of T-synthase is almost doubled in COSMC knockdown cells. c Relative mRNA expression of GalNAc-transferase isoforms in L3.6pl. d Relative quantification of T-synthase and COSMC mRNA expression in L3.6pl COSMC knockdown and control cells. e T-synthase activity was measured in Panc-1 and L3.6pl COSMC knockdown and control cells using GalNAc-α-4MU fluorescent assay