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Fig. 1 | Molecular Cancer

Fig. 1

From: A non-tight junction function of claudin-7—Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment

Fig. 1

Knockdown of claudin-7 in HCC827 lung cancer cells. a Representative phase and green fluorescence images of live control and claudin-7 KD cells. Both control and claudin-7 shRNA lentivirus constructs contain a GFP expression sequence. b Top panel is the anti-claudin-7 immunofluorescence staining of control and claudin-7 KD cells. Claudin-7 level was dramatically decreased in claudin-7 KD cells. The bottom panel is the anti-GFP immunofluorescence staining. The cells were fixed with 100 % methanol, which lead to GFP proteins leaking out of the cells so that the green fluorescence shown in the top panel was only the claudin-7 signal. c Western blot shows the diminished level of claudin-7 in the KD cells (left). The statistical analysis of claudin-7 expression in control and KD cells from five independent experiments is shown on right. *P < 0.05. d Protein expression levels of E-cadherin, claudin-1, −3, and −4 in control and KD cells. Only claudin-4 expression level was increased in KD cells. GAPDH served as a loading control

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