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Fig. 2 | Molecular Cancer

Fig. 2

From: A non-tight junction function of claudin-7—Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment

Fig. 2

Increased cell proliferation in claudin-7 KD cells. a Five × 103 control and claudin-7 KD cells were seeded into 24-well plates and the cell number was counted for each sample on days 2, 4, and 6 after plating. Claudin-7 KD cells show significantly higher proliferation rate compared to the control cells on days 4 and 6. *P < 0.05. b Three days after plating, cells were trypsinized, centrifuged, and stained with propidium iodide and subjected to cell cycle analysis by flow cytometry. Claudin-7 KD cells show a higher percentage of G2/M and S phase cells and lower percentage of apoptotic cells. *P < 0.05. c Representative western blots show increased levels of phospho-ERK1/2, survivin, and phospho-Bcl-2 and decreased level of cleaved PARP in claudin-7 KD cells when compared to control cells. A total of 30 μg proteins were loaded for each sample. d The top panel is the representative cell cycle analysis of control and claudin-7 KD cells in regular culture medium 3 days after plating. The bottom panel was taken after 24 h of aphidicolin treatment, showing that both cell lines were inhibited at S phase. e Control and KD cells were treated without (left) and with 24 h of aphidicolin (right). Cell lysates were collected 2.5 and 18 h after releasing from aphidicolin treatment

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