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Fig. 5 | Molecular Cancer

Fig. 5

From: A non-tight junction function of claudin-7—Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment

Fig. 5

Treatments of claudin-7 KD cells with collagens, genistein and aphidicolin. a Real-time RT-PCR revealed that several collagens were significantly decreased in claudin-7 KD cells when compared to the control cells. *P < 0.05. b By culturing the cells on type IV collagen-coated culture plates, claudin-7 KD cells displayed the increased cell size and were more spread out. c Five × 103 control or claudin-7 KD cells were plated on type IV collagen-coated culture plates. Cell number was counted on days 2, 4, and 6 after plating. Claudin-7 KD cells showed a higher proliferation rate than control cells. *P < 0.05. d Phase images of control and KD cells after 24 h of DMSO (control) or 200 μM genistein treatment. e Phase images of control and KD cells after 24 h of DMSO or 5 μg/ml aphidicolin treatment. f Control and KD cells cultured on the collagen IV coated plate, or treated with genistein or aphidicolin were lysed, loaded onto SDS-polyacrylamide gel, and probed with anti-integrin β1 antibody

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Molecular Cancer

ISSN: 1476-4598