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Fig. 6 | Molecular Cancer

Fig. 6

From: A non-tight junction function of claudin-7—Interaction with integrin signaling in suppressing lung cancer cell proliferation and detachment

Fig. 6

Rescued claudin-7 KD cell defects by exogenous expression of claudin-7 or integrin β1 in KD cells. a The claudin-7 construct tagged with myc was transfected into the HCC827 claudin-7 KD cells. The transfected claudin-7 sequence has three nucleotide differences from lentivirus claudin-7 shRNA (due to mouse and human claudin-7 difference), so it is resistant to the lentivirus shRNA. The claudin-7 KD cells with claudin-7-myc transfection became larger in size and more spread out (phase images). Anti-myc immunofluorescence staining indicated by arrows shows the exogenous claudin-7 expression in claudin-7 KD cells. b Lysates from claudin-7 KD cells transfected with vector (KD + V) or claudin-7-myc (KD + 7) were collected and subjected to western blot analysis. The expression levels of integrin β1 and cleaved PARP were both increased in claudin-7 transfected KD cells. The membrane probed with anti-myc antibody shows the exogenous claudin-7 expression in KD cells. c Five × 103 claudin-7 KD cells transfected with vector or claudin-7-myc were plated into 24-well plates. Cell number was counted on days 1, 3, 5 after plating. *P < 0.05. d Claudin-7 KD cells transfected claudin-7 (upper panel) or integrin β1 (lower panel) construct were trypsinized and plated on uncoated glass coverslips. e Lysates from claudin-7 KD cells transfected with vector (KD + V) or integrin β1 (KD+ β1) were collected and subjected to western blot analysis. The membrane probed with anti-integrin β1 antibody shows the expression level of integrin β1 in KD cells without or with integrin β1 transfection

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