Fig. 7
The inhibitory effect of claudin-7 on tumor growth in nude mice. a Two × 106 HCC827 control or claudin-7 KD cells were harvested and suspended in the culture medium, then subcutaneously injected into the left and right flanks of a total of 10 nude mice, respectively. Arrows indicate the tumors generated subcutaneously in the representative nude mouse which was sacrificed 30 days post tumor cell inoculation. b Western blot analysis shows the increased expression levels of phospho-ERK1/2, survivin, and cleaved PARP in claudin-7 KD cells-induced tumors when compared to those of control cells-induced tumors. c, d The nude mice were sacrificed and the tumors were removed and measured in both volume and weight. Statistical analysis was conducted on a total of 10 nude mice. Medians were shown as black solid lines in the figures. *P < 0.05. e, f Growth curves for ten control cells-and ten claudin-7 KD cells-inoculated tumors