Figure 3

Twist and Snail distribution in 1833 cells in response to HGF and TGFβ signallings. a The cells were exposed to HGF or TGFβ1 for various times on coverslips, with or without WWOX e.v. or siRNAWWOX, and were probed with anti-Twist (green) or anti-Snail (red) antibody. The nuclei were stained with DAPI (blue). The panels 1, 2, 3 show the merge images: 1 = green/DAPI; 2 = red/DAPI; 3 = green/red. b The cells exposed to 4-h HGF on coverslips, were probed with anti-pTwist1 (magenta) antibody; the nuclei were stained with DAPI, and their merge images are shown. For a and b the images, taken at 400× magnification, are representative of experiments performed in triplicate. c Representative images of Western blots repeated three times are shown, and the phosphoAMPK/AMPK ratio is indicated. The ratios were calculated using the densitometric values, obtained by taking into consideration all the specific-protein bands in each lane. Vinculin was used for normalization. d The cells, transfected with the 3TPLux gene reporter, were exposed to HGF in the presence or the absence of Compound C. The data are the means ± S.E. of three independent experiments performed in triplicate. ***P < 0.001 versus basal luciferase activity; ^ΔΔΔP < 0.001 versus the luciferase activity under HGF