Fig. 6

HIF-1 transactivating activity and its DNA binding, and TGFβ1 transactivating activity under WWOX expression vector. a Representative images of Western blots repeated three times are shown. Vinculin was used for normalization. The numbers at the bottom indicate the fold variations relative to control, considered as 1. b The cells, transfected with the gene reporter driven by the HRE multimer, were co-transfected with WWOX e.v. in the presence of the absence of ΔTwist. The data are the means ± S.E. of three independent experiments performed in triplicate. *P < 0.05, **P < 0.005 versus basal luciferase activity; ^ΔΔΔP < 0.001 versus luciferase activity under WWOX e.v. c Nuclear extracts from control or WWOX e.v.-transfected cells were used for DNA-binding assay, and super-gelshift with the indicated antibodies was done. 50× competition was performed with an excess of unlabeled oligonucleotide. const, constitutive binding. A representative image of three independent experiments is reported. d The cells, transfected with the construct containing the entire TGFβ1 promoter or its fragment (see Scheme), were co-transfected with WWOX e.v. in the presence or the absence of ΔTwist. The data are the means ± S.E. of three independent experiments performed in triplicate. **P < 0.005, ***P < 0.001 versus respective basal luciferase activity; ^ΔP < 0.05 versus the luciferase activity in response to WWOX e.v.; °P < 0.05 versus the luciferase activity in the presence of ΔTwist