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Fig. 4 | Molecular Cancer

Fig. 4

From: The chemokine receptor CCR6 facilitates the onset of mammary neoplasia in the MMTV-PyMT mouse model via recruitment of tumor-promoting macrophages

Fig. 4

CCR6 promotes mammary gland neoplasia independently of cancer stem-like cells a-b Single cell suspensions (CD45-negative) from mammary glands with early neoplasia from MMTV-PyMT Ccr6 WT and Ccr6 −/− mice were analyzed by flow cytometry for the expression of CD24 and CD29 to quantitate the proportions of basal and luminal cell lineages. c Mammosphere-forming efficiency of cells isolated from MMTV-PyMT Ccr6 WT and Ccr6 −/− mammary glands. a-c Data are representative of 3 independent experiments, n = 6 mice per genotype per experiment. d Mammosphere-forming efficiency of MMTV-PyMT Ccr6 WT cells stimulated with varying concentrations of CCL20. Data are representative of 3 independent experiments, n = 3 individual mice per experiment. e Representative whole mount images and quantitation of the neoplastic outgrowth area in Ccr6 WT recipients of MMTV-PyMT Ccr6 WT and MMTV-PyMT Ccr6 −/− mammary tumor tissue transplants; n = 4 recipient mice per group, LN = lymph node. Black arrowheads indicate donor transplant outgrowth within the glands

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Molecular Cancer

ISSN: 1476-4598