Fig. 4
A-1155463 sensitivity correlates with the effect of BCL-XL knockdown and apoptosis. BCL2L1 or control siRNA was transfected into two A-1155463-sensitive cell lines (LS1034 and SW1417) and two resistant cell lines (GEO and RKO). a Viability was determined after 72 h by microscopy. Western blotting showing the degree of BCL-XL knock-down by BCL2L1 siRNAs. Levels of BCL-XL knockdown were measured by densitometry and shown as BCL-XL/actin. b Viability was determined after 72 h with CellTiter Glo reagent. c BCL-XL inhibitor induces apoptosis through activation of caspase-3/7 pathway. LS1034 and SW1417 cells were treated with DMSO or 200 nM A-1155463, with or without 50 μM Z-VAD caspase inhibitor for 24 h. DNA content was measured by flow cytometry to determine the effect of the inhibitors on the cell cycle and cell death. The percentage of cells in each phase of the cell cycle was plotted