Fig. 4

Anti-tumor effects in tumor xenografts upon inhibition of PBK through administration of HI-TOPK-032. a T08 tumor cells were subcutaneously transplanted in the right flank of SCID mice. After establishment of tumors, mice were treated three times weekly by intra-tumoral injection of (i) control and (ii) HI-TOPK-032 (5 and 10 mg/kg) suspensions. The tumors were extracted after 4 weeks of treatment and a representative tumor from each group is shown. b Mean tumor volume was calculated. Error bars = SD. Mean tumor volumes of HI-TOPK-032 treated tumors (5 and 10 mg/kg) were significantly different than the control group (p < 0.05 and p < 0.005 respectively). c HI-TOPK-032 had no effect on mouse body weight. Mice in both treated and untreated groups were weighed once a week. Error bars = SD (d, e). Immunofluorescence analysis of HI-TOPK-032 (10 mg/kg) treated and control tumor cryosections stained for Ki-67 and cleaved caspase-3 expression. Scale bar = 20 μm