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Fig. 1 | Molecular Cancer

Fig. 1

From: Exosome-mediated microRNA signaling from breast cancer cells is altered by the anti-angiogenesis agent docosahexaenoic acid (DHA)

Fig. 1

Isolation and molecular characterization of exosomes shed from breast cancer cells. a Exosomes were isolated from the conditioned media of control or treated MCF7 cells (100 μM DHA for 24 h) using the Exoquick Reagent, fixed, whole-mounted on coated Formvar grids, negatively stained and visualized by electron microscopy (Hitachi H-7600). Left column, 40,000×; right column 100,000×. Images are representative of three experiments. b Immunogold labeling and electron microscopy of human CD63 (10 nM gold particles) on exosomes isolated from MCF7 cells (100,000×). c Western blot analysis of the exosome marker CD63 (30–60 kDa) in exosomes shed from MCF7 and MDA-MB-231 cells. Exosome lysates were run under non-reducing conditions. d RNA Agilent Bioanalyzer profile of total RNA extracted from control and DHA treated MCF7 cells and their purified exosomes. The peak at 25 nt is a lower marker standard and is indicated on the upper left image. In the exosome preparations the RNA is predominantly <1,000 nucleotides in length, with a large peak noted in the small RNA range of the DHA-treated exosomes and the 28S and 18S ribosomal subunit RNAs (as indicated on upper left image) are notably absent from both treated and untreated exosome preparations

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