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Fig. 2 | Molecular Cancer

Fig. 2

From: Bergapten drives autophagy through the up-regulation of PTEN expression in breast cancer cells

Fig. 2

Bergapten transactivates PTEN promoter gene in MCF-7 and ZR-75 cells. a Left panel: schematic representation of deletion fragments of the PTEN gene promoter. Right panel: constructs depicted were transiently transfected in MCF-7 and ZR-75 cells as indicated, treated with vehicle (−), Bg 20 μM or Bg 20 μM + SB203580 (10 μM). b Site-directed mutagenesis of the NF-Y site. pGL3-612 and pGL3-612 Mut promoter constructs were transfected into MCF-7 cells, and promoter activity was assessed in the absence or presence of Bg 20 μM treatment. Columns are mean of three independent experiments and expressed as fold change over vehicle, which was assumed to be 1; bars, SD; *P < 0.05 vs vehicle. ** P < 0.05 vs pGL3-612 Bg 20 μM. c Western blot analysis of PTEN and NF-Y in MCF-7 cells transfected with non specific (NS) or targeted against NF-Y siRNA treated with vehicle (−) or Bg 20 μM for 16 h. β-actin was used as loading control. Autoradiographs show the results of one representative experiment out of three. Columns, are mean of three independent experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as fold over vehicle, which was assumed to be 1; bars, SD;* P < 0.05 vs NS siRNA vehicle. ** P < 0.05 vs NS siRNA Bg 20 μM

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