Fig. 3

Bergapten modulates the expression of proteins involved in autophagy process in MCF-7 and ZR-75 cells. a Western blot analysis of PTEN (also showed in Fig. 1b) and Beclin1 in MCF-7 cells transfected with non specific (NS) or targeted against PTEN siRNA treated with vehicle (−), Bg 20 μM or Bg 50 μM for 16 h. β-actin was used as loading control. Autoradiographs show the results of one representative experiment. Columns, are mean of three independent experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as fold change over vehicle, which was assumed to be 1; bars, SD;* P < 0.05 vs NS siRNA vehicle. ^ P < 0.05 vs NS siRNA Bg 20 μM. ** P < 0.05 vs NS siRNA μM. b Western blot analysis of PI3KIII, UVRAG and AMBRA expression in MCF-7 and ZR-75 cells treated for 16 h with vehicle (−), Bg 20 μM, Bg 50 μM. Columns, are mean of three independent experiments in which band intensities were evaluated in terms of optical density arbitrary units and expressed as fold over vehicle, which was assumed to be 1; bars, SD; *P < 0.05 vs vehicle treated cells. c Time course study. Western blot analysis of p62 and LC3 expression in MCF-7 and ZR-75 cells treated for different times as indicated with vehicle (−), Bg 20 μM, Bg 50 μM. Autoradiographs show the results of one representative experiment out of three