Fig. 4
From: Bergapten drives autophagy through the up-regulation of PTEN expression in breast cancer cells
Bergapten treatement increases autophagosome formation in MCF-7 and ZR-75 cells. a MCF-7 cells were transiently transfected with LC3-GFP and treated with vehicle (−) or with Bg 20 μM or Bg 50 μM for 16 h. b MCF-7 and ZR75 cells treated with vehicle (−) or with Bg 20 μM or Bg 50 μM for 16 h, were incubated with acridine orange and fluorescence was analyzed by flow cytometry. c MCF-7 transfected with non specific (NS) or targeted against NF-Y siRNA treated with vehicle (−) or with Bg 20 μM for 16 h. Images show the results of one representative experiment out of three