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Fig. 1 | Molecular Cancer

Fig. 1

From: Epiregulin contributes to breast tumorigenesis through regulating matrix metalloproteinase 1 and promoting cell survival

Fig. 1

Regulation of EREG expression in MCF10DCIS cells by FGFR activity. a qRT-PCR of the indicated EGF ligands was performed on RNA isolated from MCF10A cells and MCF10DCIS cells. Expression levels were normalized to levels of CYBP. b Soluble levels of EREG in conditioned media obtained from MCF10A and MCF10DCIS cells as determined by ELISA. c qRT-PCR of the EREG expression was performed on RNA isolated from the indicated cell lines. d Immunoblot analysis was performed to examine the effects of the indicated amounts of dovitinib on phosphorylation of FRS-2 in MCF10DCIS cells. e Concentration of EREG in conditioned media, as determined by ELISA, from MCF10DCIS cells treated with the indicated amounts of dovitinib for 18 h. f qRT-PCR analysis of FGF-2 expression in MCF10A and MCF10DCIS cells. Levels normalized to CYBP. *p < 0.05, **p < 0.001, ***p < 0.0001

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