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Fig. 3 | Molecular Cancer

Fig. 3

From: Epiregulin contributes to breast tumorigenesis through regulating matrix metalloproteinase 1 and promoting cell survival

Fig. 3

Decreased expression of EREG leads to reduced tumor growth in vivo. a 50,000 MCF10DCIS-shNT, MCF10DCIS-shEREG1 or MCF10DCIS-shEREG1 cells were injected subcutaneously into nude mice. Once tumors reached 100 mm3, mice were provided doxycycline. Tumors were measured every other day. b Tumors harvested, sectioned and stained with hematoxylin and eosin (H&E). TUNEL analysis was performed to identify apoptotic cells (green). Scale bars represent 50 μm. c Immunoblot analysis was performed on protein samples isolated from pooled tumors to detect pEGFR. Immunoblotting of total EGFR was performed to assess equal loading. d Quantification of TUNEL-positive cells in tumors from (a). n = 4 tumors per construct. *p < 0.05, **p < 0.005, ***p < 0.0001

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