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Fig. 5 | Molecular Cancer

Fig. 5

From: Epiregulin contributes to breast tumorigenesis through regulating matrix metalloproteinase 1 and promoting cell survival

Fig. 5

MMP-1 contributes to EREG-induced survival of MCF10A cells. a qRT-PCR analysis of MMP-1 gene expression in MCF10A and MCF10AT cells treated with either solvent (PBS) or 10 ng/ml rhEREG. Expression levels of MMP-1 were normalized to levels of CYBP. b Immunoblot analysis of MMP-1 protein in conditioned media obtained from MCF10A and MCF10AT cells following 24 h of rhEREG treatment. Loading was assessed by Coomassie staining (Additional file 1: Figure S1A). c Immunoblot analysis of MMP-1 protein in conditioned media obtained from MCF10A cells treated with either non-targeting (NT) or MMP-1 siRNA following 24 h of rhEREG treatment. Loading was assessed by Coomassie staining (Additional file 1: Figure S1B). d Quantification of acinar diameter of structures. MCF10A cells were transfected with either NT or MMP-1 siRNA prior to plating in recombinant basement membrane with either solvent (PBS) or 10 ng/ml rhEREG. e Quantification of apoptotic structures. Structures that were positive for at least one cleaved caspase-3 positive cell were counted. f MCF10A cells were treated with non-targeting (NT) or MMP-1 siRNA, serum starved and treated with solvent control (PBS) or with 10 ng/ml rhEREG in the presence or absence of 2 μM doxorubicin (Dox). Effects on apoptosis were assessed by immunoblot analysis for cleaved caspase-3. β-tubulin is shown as a loading control. *p < 0.05, **p < 0.001, ***p < 0.0001

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