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Fig. 3 | Molecular Cancer

Fig. 3

From: Hypoxia inducible prolyl hydroxylase PHD3 maintains carcinoma cell growth by decreasing the stability of p27

Fig. 3

PHD3 elevates p27 expression through a post-translational mechanism. a PHD3 depletion has no effect on p27 transcription under hypoxia. p27 mRNA levels were measured in HeLa cells using quantitative real-time PCR. Results shown as fold change vs normoxic control, four independent experiments (± SEM) (p = n.s.; n = 4). b Hypoxic p27 expression is HIF-1α and EPAS1/HIF-2α independent. QRT-PCR analysis of p27 and hypoxia-inducible glut-1 mRNA normalized to β-actin using the indicated double knockdown after 24 h of hypoxia. Unlike glut-1 HIF knockdown has little effect on p27 transcription. Results from three independent experiments (±SEM) are shown (p = n.s.; n = 3). c PHD3 depletion induces p27 protein levels independently of HIF-1α or EPAS1/HIF-2α depletion in HeLa cells. d Quantification of p27 protein expression using indicated double knockdown. Results from three independent experiments (±SEM) are shown

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