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Fig. 3 | Molecular Cancer

Fig. 3

From: AduPARE1A and gemcitabine combined treatment trigger synergistic antitumor effects in pancreatic cancer through NF-κB mediated uPAR activation

Fig. 3

Analysis of the effects of combined treatments on AduPARE1A production and the level of apoptotic cell death in BxPC-3 and PANC-1 cell lines. a AduPARE1A viral production in BxPC-3 and PANC-1 cell lines at 72 h after infection. Cell lines were infected with AduPARE1A (4000 vp/cell; 200 vp/cell, BxPC-3 and PANC-1 respectively) and 4 h later the medium was removed and treated, or not, with Gemcitabine (50 ng/ml; 500 μg/ml, BxPC-3 and PANC-1 respectively) for 72 h. Viral DNA obtained from whole cell extracts at 4 and 72 h was analyzed for hexon amplification by qPCR. Values are represented as a mean ± SEM of four independent experiments (*P < 0.05). b and c Western blot of cleaved caspase-3 (b) and cleaved PARP (c) in BxPC-3 and PANC-1 cell lines treated with mock, gemcitabine (50 ng/mL; 500 μg/mL, BxPC-3 and PANC-1 respectively), AduPARE1A (4000 vp/cell; 200 vp/cell, BxPC-3 and PANC-1 respectively) or AduPARE1A plus gemcitabine at 48 h after treatment. Bar graphs show quantification of cleaved caspase-3 and cleaved PARP related to GAPDH expression in n = 5 (BxPC-3) and n = 6 (PANC-1) independent experiments. (*P < 0.05, **P < 0.01)

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