Fig. 2
From: Stratifin accelerates progression of lung adenocarcinoma at an early stage
Generation of Tg-SPC-SFN+/−. a A cDNA for SFN was inserted between the SPC promoter and both the splicing and polyadenylation (polyA) signal sequences. b RT-PCR using cDNA extracted from lung, heart, kidney, liver, and spleen of representative Tg-SPC-SFN+/− mice. c hSFN IHC using FFPE specimens of each of the organs from representative Tg-SPC-SFN+/− mice. Transgene expression (hSFN) was detected only in lung. Magnification: ×200. d CT image of representative Tg-SPC-SFN+/− lungs 10, 15, and 20 weeks after NNK administration. Arrows indicate suspected tumors. Scale bar: 10 mm. e Gross appearance of lungs of Tg-SPC-SFN+/− mice administered NNK (1) and saline (2). Arrows indicates tumors. Scale bar: 5 mm. f hSFN IHC using FFPE specimens of tumors and adjacent normal lung from Tg-SPC-SFN+/−mice. Magnification: ×200