Fig. 3

Antibody-induced phenotypes in 3-D cultured PC-3 cells in a high content screen. a Clustering of antibody-induced phenotypes in a high content screen performed using PC-3 cells grown in a 3D matrix. Each antibody was dosed at six different concentrations and in four replicates. Phenotypic measurements were determined from images and analysed as described in Methods. The replicates were averaged prior to clustering analysis, but different doses of each antibody were analysed separately. The dendrogram shows the clustering pattern of individual samples into 5 phenotypic classes, A–E (see also Additional file 5: Table S1. The heat map represents the values for six phenotypic features that distinguished these classes (red = positive; blue = negative)–see Methods for definitions of these features. b Bar chart showing the contributions of the six phenotypic features, either positively or negatively, to the five phenotypic classes used to assign antibody-induced effects on the PC-3 cells