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Fig. 4 | Molecular Cancer

Fig. 4

From: Identification of anti-tumour biologics using primary tumour models, 3-D phenotypic screening and image-based multi-parametric profiling

Fig. 4

Opposing effects of two groups of anti-CDCP1 molecules upon PC-3 cell invasiveness. CDCP1-binding DARPin-Fcs segregate into two populations in the phenotypic space determined by the multiparametric high-content screen performed on PC-3 cells: plots showing the effects on cell polarity (a) and invasion inhibition (b) . Group 1 (blue symbols/lines) behave similarly to αCDCP1-Ab1 (in purple), inhibiting invasion and somewhat increasing cell polarity. Group 2 (in reds) decrease polarity, and increase invasiveness. Control antibodies are shown in green. c Scatter plot showing a correlation between cell polarity and invasion inhibition for the same dataset as in panels a/b. Each point represents a single concentration of an antibody, using the same colour scheme as in (a/b). d Example images from the high content screen, showing the morphological effects of different classes of anti-CDCP1 antibodies on PC-3 cells grown in 3D culture. Each row shows image stacks of cells cultured in the presence of an anti-invasive anti-CDCP1 (top row) pro-invasive anti-CDCP1 antibody (middle row) or negative control antibody (bottom row), at increasing concentration from left to right. The fourth column shows an enlarged view of the indicated areas. Staining: red = filamentous actin, blue = nuclei

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