Fig. 5

Glycosylated αDG and βDG immunoreactivity is frequently reduced in clear cell renal cell carcinoma. Samples stained for either αDG (IIH6) or βDG (8D5) were scored according to a quartile scoring system whereby 3 = positive (≥90 % of cells intensely positive); 2 = heterogeneous (regional positivity with >10 % of cells negative); 1 = reduced (>10 % of cells negative and decreased intensity of staining); and 0 = loss (≤1 % of cells positive). a–d. αDG staining and associated scores. e–h. βDG staining and associated scores. i, j. Normal tissue demonstrating positive staining. k. αDG and βDG staining score distribution. l. Scores were grouped by Fuhrman nuclear grade and demonstrate no significant association between staining and grade