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Fig. 4 | Molecular Cancer

Fig. 4

From: A critical role of Oct4A in mediating metastasis and disease-free survival in a mouse model of ovarian cancer

Fig. 4

Loss of Oct4A expression resulted in reduced adhesion abilities of spheroids and colony formation in HEY cells. a Vector control and Oct4A KD cells were grown as non-adherent spheroids cultures on ultra-low attachment plates as described in the Material and Methods. Following 18 day incubation, spheroids were transferred to 6 well plastic culture plates and incubated for 24 h. Adhered spheroids were then fixed with formalin and stained with 5 % crystal violet. All images are representative of three independent experiments performed in duplicate. Magnification 100x and scale = 100 μM b Colony formation in 18 day Oct4A KD spheroids was significantly reduced when compared to vector control spheroids. c Expression of adhesion markers CD44 and EpCAM in monolayer Oct4A KD cells was determined by qPCR analysis. Relative quantification CD44 and EpCAM mRNA expression in Oct4A KD cells were standardized to 18S housekeeping gene and normalized to the vector control. All data sets are presented as the mean ± SEM of three independent experiments as determined by One-Way ANOVA and Dunnett's Multiple Comparison post-test compared to vector control. Significance is indicated by *P < 0.05, **p < 0.01 and ***P < 0.001

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