Fig. 6
In vivo tumour development in mice injected with HEY Oct4A knockdown cells. a 5 × 106 vector control, Oct4A KD1 and Oct4A KD2 HEY cells were inoculated by ip injection into 6–8 week old female BALB/c nu/nu mice (n = 11/group). 4 weeks post injection tumour development was photographed, mice euthanized and b tumour burden calculated. Oct4A KD cells exhibited significantly decreased tumour formation as determined by One Way ANOVA and Turkey’s post-test. Significance is indicated by ***p < 0.001. c Excised tumours were subjected to H&E staining. Images are set at 200x magnification and are representative of n = 4 per group. Scale bar is set at 50 μM. d Prolonged survival status in mice injected with Oct4A KD cells. n = 8 mice per Oct4A knockdown group were maintained over a 6 month period and monitored for tumour development. Mice were culled and death reported as mice reached predetermined endpoint criteria (Refer to Methods and Materials for list of criteria). Mice injected with Oct4A KD cells displayed significant increase in mean survival as determined by Kaplan-Meier survival analysis