Fig. 5
From: Dioxin receptor regulates aldehyde dehydrogenase to block melanoma tumorigenesis and metastasis
Aldh1a1 knockdown blocks primary tumorigenesis and lung metastasis induced by AhR depletion. a, b B16F10 and sh-Aldh1a1 cells were injected subcutaneously in the dorsal flank of AhR+/+ immunocompetent mice. Each cell line was injected in one of the two flanks of each mouse. Tumors were collected, weighted (a) and their volume (b) calculated using the formula [length x width2 x 0.4]. A total of 9 and 8 tumors were analyzed for B16F10 and sh-Aldh1a1 cells, respectively. c, d sh-AhR and sh-AhR + sh-Aldh1a1 cells were injected subcutaneously in the dorsal flank of AhR+/+ immunocompetent mice. Each cell line was injected in one of the two flanks of each mouse. Tumors were collected, weighted (c) and their volume (d) calculated using the formula [length × width2 × 0.4]. A total of 12 and 14 tumors were analyzed for sh-AhR and sh-AhR + sh-Aldh1a1 cells, respectively. e For the analysis of metastasis, both cell lines were inoculated through the tail vein in the same strain of recipient mice indicated above. Lungs were removed and the number of metastatic nodules counted and represented. Eight mice were injected for each cell line. The B16F10 cell line was also transduced with a retrovirus containing the empty vectors used to produce the sh-Aldh1a1 cell line. Data are shown as mean ± SD. n.s. = statistically non-significant