Fig. 6

Reduced Aldh1a1 expression blocks the metastatic burden of AhR depletion in melanoma cells. a sh-AhR:LUX and sh-AhR + sh-Aldh1a1:LUX cells, stably expressing the luciferase protein, were injected through the tail vein into C57BL6 albino mice. Mice were injected i.p. with luciferin at the time of cell inoculation and after 2, 7, 11, 14 and 21 days and analyzed using an IVIS equipment. b Metastatic dissemination was quantified by measuring light emission and the results have been represented as photons/second for each cell line. c Kaplan-Meier plot for the survival of mice injected with sh-AhR:LUX or sh-AhR + sh-Aldh1a1:LUX cells. A total of 10 C57BL6 albino mice were used for each experimental condition. Data are shown as mean ± SD