Skip to main content
Fig. 2 | Molecular Cancer

Fig. 2

From: Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF

Fig. 2

Differentiation of GBM CSCs using BMP4 leads to upregulation of differentiation markers and downregulation of the CSC marker CD133 which is reversed by ECCM. a BMP4 induces upregulation of the astrocyte marker GFAP in G073 (left) and G062 (right) cells and induction of the neuronal marker βIII-tubulin in G073 cells as compared to cells plated in CSC medium + GFs (scale bars 20 μm). b-d Upper panels: G073, lower panels: G062. b Differentiation markers are upregulated and CSC markers are downregulated upon BMP4 treatment compared to cells plated in CSC medium + GFs as determined by qRT PCR (1 representative of 3 independent experiments is shown) and c the CSC marker CD133 is not detectable anymore (n > 3). d BMP4-differentiated CD133 cells were sorted and plated in either control medium or ECCM. 5 days after sorting CD133 expression was reanalyzed by FACS (n ≥ 4)

Back to article page