Fig. 2
From: Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF
Differentiation of GBM CSCs using BMP4 leads to upregulation of differentiation markers and downregulation of the CSC marker CD133 which is reversed by ECCM. a BMP4 induces upregulation of the astrocyte marker GFAP in G073 (left) and G062 (right) cells and induction of the neuronal marker βIII-tubulin in G073 cells as compared to cells plated in CSC medium + GFs (scale bars 20 μm). b-d Upper panels: G073, lower panels: G062. b Differentiation markers are upregulated and CSC markers are downregulated upon BMP4 treatment compared to cells plated in CSC medium + GFs as determined by qRT PCR (1 representative of 3 independent experiments is shown) and c the CSC marker CD133 is not detectable anymore (n > 3). d BMP4-differentiated CD133− cells were sorted and plated in either control medium or ECCM. 5 days after sorting CD133 expression was reanalyzed by FACS (n ≥ 4)