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Fig. 4 | Molecular Cancer

Fig. 4

From: Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF

Fig. 4

bFGF in ECCM is responsible for induction of CD133 expression and increases the clonogenic potential of BMP4-differentiated CD133 cells. a BMP4-differentiated CD133 G073 cells were sorted and plated in control medium, ECCM, ECCM containing a bFGF neutralizing antibody (bFGF nAb), or ECCM containing Bevacizumab. 5 days after sorting CD133 expression was reanalyzed by FACS (n = 3). b FACS plots corresponding to (a). c The clonogenic potential of BMP4-differentiated CD133 G073 cells was assessed using clonogenic assays (n = 6). d CD133 expression of spheres formed in (c) in the bFGF condition compared to the non-differentiated parental G073 spheroid culture (n = 2). e The clonogenic potential of BMP4-differentiated CD133 G062 cells was assessed using limiting dilution assays (n = 5)

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