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Fig. 5 | Molecular Cancer

Fig. 5

From: Endothelial cells induce cancer stem cell features in differentiated glioblastoma cells via bFGF

Fig. 5

Differentiated GBM oligodendrocytes can be phenotypically and functionally redirected to the CSC state by bFGF. a Differentiation of G073 cells for 7 days by GF withdrawal (− GFs). Differentiation induces morphological changes and strong upregulation of the oligodendrocyte marker O4 (scale bars 20 μm). b Analysis of differentiation and CSC markers by qRT PCR. Depicted is the fold change compared to cells plated in CSC medium + GFs. 1 representative of 3 independent experiments is shown. c Differentiated GBM cells express the oligodendrocyte marker O4 on their surface as determined by FACS. d CD133O4+ cells were sorted and plated in the indicated condition. 5 days after sorting CD133 and O4 expression were reanalyzed by FACS (n = 3). e The clonogenic potential of differentiated CD133O4+ cells was determined using clonogenic assays (n = 4). f CD133 and O4 expression of spheres formed in (e) in the bFGF condition compared to the non-differentiated parental GBM spheroid culture (n = 3)

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