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Fig. 2 | Molecular Cancer

Fig. 2

From: Bidirectional crosstalk between PD-L1 expression and epithelial to mesenchymal transition: Significance in claudin-low breast cancer cells

Fig. 2

Epithelial to mesenchymal transition (EMT) upregulates PD-L1 in the human breast cells. a Bar graphs showing the expression level of PD-L1 as measured by flow cytometry in four normal breast cells before and after EMT induction using continuous treatment with TGF-β1 (2.5 ng/mL) for 7 days for MCF-12A and MCF10A cells and 14 days for primary luminal and HMLE cells. Bars represent the means of 3 independent experiments ± standard deviation (SD) b Representative flow cytometry histograms for PD-L1 and c a typical image for cells before and after EMT induction. d representative flow cytometry histograms showing the expression of intracellular vimentin/E-cadherin molecules and dot blots of CD44/CD24 cell surface markers of MCF12A after EMT induction using TGF-β1 (2.5 ng/mL)

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