Fig. 5

Manipulation of PD-L1 modulates EMT status of breast cancer cells. EMT status determined by the expression of CD44, CD24, and vimentin or CD44/CD24 combination as measured by flow cytometry following PD-L1 knockdown using specific Sh-RNA mesenchymal-like (MDA-MB-231) breast cancer cells (a & b). or PD-L1 overexpression by transfection with PD-L1 ORF in the luminal-like (T47D) breast cancer cells (c). Bars in a represent the means of 3 three different clones and 3 different experiments ± standard deviation (n = 9) while histograms in b & c are representative of one of the experiments. Lines in each histograms represent threshold of positivity as determined by isotype control except of CD44 in MDA-MB-231 cells where it is an arbitrary line to show CD44^high status