Fig. 7

Knockdown of SRSF3 changes splicing pattern of KMT2C and decreases methylated H3K4. a RT-PCR of KMT2C transctipts between exon 44 and exon 46. The numbers in the middle of the image indicate the DNA bands. The diagram of the alternative splicing events is shown on the right. The right and left arrows over exons 44 and 46 represent the primer pair used for PCR. b Detection of monomethylated H3K4 and trimethylated H3K4, H3K9 and H3K27 by western blotting. Unmodified histone H3 was a loading control. c Quantitation of western blotting results in (b). The levels of modified H3 were first normalized to the levels of unmodified H3 and then the relative levels of modified H3 were calculated using the samples without Doxy treatment as the references