Fig. 2
From: Retinoic acid-induced CHD5 upregulation and neuronal differentiation of neuroblastoma
Upregulation of CHD5 expression in NB cells treated with 13cRA. (a) Representative immunoblot showing the upregulation of CHD5 from nuclear extracts of SY5Y cells treated with 13cRA at different concentrations and times. Western blot was stripped and probed with HDAC2 as an internal control for nuclear proteins, and we observed uniform HDAC2 expression. (b) and (c) Immunoblotting of CHD5. NB cell lines SY5Y, NGP, SK-N-DZ, IMR5, LAN5 and SK-N-FI were treated in the presence or absence of 10 μM 13cRA for 12 days and nuclear proteins were extracted. Western analysis showed CHD5 protein upregulation in SY5Y, NGP and SK-N-DZ cells (b), but not in IMR5, LAN5 and SK-N-FI (c). HDAC2 protein was used as an internal control for these blots