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Fig. 1 | Molecular Cancer

Fig. 1

From: CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA

Fig. 1

CD90+ population. a Huh7 and sorted CD90+ Huh7 were stained for hepatocytic (HNF4alpha), epithelial (E-Cadherin) and mesenchymal (Vimentin) markers, in blue the nuclear staining with DAPI. Characterization of isolated exosomes. b Dynamic light scattering of vesicles isolated from Huh7 (in black) and from CD90 + Huh7 cells (in red). c Western blot for endosomal markers Alix, Tsg101 and HSC70 in Huh7 and CD90+Huh7 population with their relative exosomes. d Confocal microscopy analysis on HUVECs treated for 1, 3, and 6 h with 5 μg/ml of exosomes from CD90+ or Huh7 cells. HUVECs were stained with phalloidin Alexa Fluor488 (green), nuclear counterstaining was done using DAPI (blue), exosomes were labelled with PKH26 (red)

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Molecular Cancer

ISSN: 1476-4598