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Fig. 3 | Molecular Cancer

Fig. 3

From: CD90+ liver cancer cells modulate endothelial cell phenotype through the release of exosomes containing H19 lncRNA

Fig. 3

a. LncRNAs expressed in CD90+ cells and their exosomes (left and middle panel). Data are expressed as fold induction compared with Huh7 mix population. Of the 90 lncRNAs analyzed, only those over-expressed more than ten-fold in CD90+ cells were considered. Listed on the right the lncRNA up-regulated in HCC. Right panel: LncRNA Profile in exosomes released by CD90 + Huh7. Data are expressed as fold of induction compared with exosomes from Huh7 parental cells. b H19 analysis. Real-time PCR analysis for H19 expression in exosomes derived from Huh7 or CD90+ cells. Exosomes were treated with RNase and subsequently processed for RNA extraction and retrotrascription. Data were normalized for β-actin and ΔΔct indicated as fold of induction compared with Huh7-derived exosomes. ***p < 0.001. c Real-time PCR for H19 on HUVEC 18 h after treatment with CD90 + exo or Huh7exo. Data were normalized for β-actin and ΔΔct indicated as fold of induction compared with control (untreated cells). ***p < 0.001

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Molecular Cancer

ISSN: 1476-4598