Fig. 4

H19 overexpression. a Left panel: Real-time PCR performed on HUVECs 18 h post-transfection. Data were normalized for β-actin and ΔΔct expressed as fold of induction pH19 vs. pEmpty **p < 0.01; *p < 0.05. Right panel: ELISA assay for VEGF level in supernatant from HUVECs 18 h after transfection. ***p < 0.001. b Left Panel: Phase contrast (20×) of tubulogenesis assay performed 18 h after transfection. Right panel: quantification of matrigel assay expressed as length of cable as arbitrary unit*p<0.05. c FACS analysis for ICAM expression in HUVEC transfected cells. d Left Panel: Phase contrast micrographs (20×) showing the adhesion of CD90 + cells on HUVEC monolayer transfected with pEmpty or pH19. Right Panel Quantification was calculated by counting the number of adherent CD90+ cells (violet) per field. **p < 0.01