Fig. 1

Expression of MIEN1 in breast cancer patient specimens and cultured cell lines. a Expression of MIEN1 in different subtypes of breast cancer patients as obtained by analyzing TCGA dataset from Oncomine. Legends and number of tissues analyzed are in parentheses- 0. No value (normal) (n = 61); 1. Apocrine Breast Carcinoma (n = 1); 2. Breast Large Cell Neuroendocrine Carcinoma (n = 1); 3. Ductal Breast Carcinoma (n = 1); 4. Intraductal Cribriform Breast Adenocarcinoma (n = 3); 5. Invasive Breast Carcinoma (n = 76); 6. Invasive Cribriform Breast Carcinoma (n = 1); 7. Invasive Ductal Breast Carcinoma (n = 392); 8. Invasive Ductal and Lobular Carcinoma (n = 3); 9. Invasive Lobular Breast Carcinoma (n = 36); 10. Invasive Papillary Breast Carcinoma (n = 1); 11. Male Breast Carcinoma (n = 3); 12. Metaplastic Breast Carcinoma (n = 1); 13. Mixed Lobular and Ductal Breast Carcinoma (n = 7); 14. Mucinous Breast Carcinoma (n = 4); 15. Papillary Breast Carcinoma (n = 1); 16. Pleomorphic Breast Carcinoma (n = 1). b MIEN1 mRNA expression in different molecular subtypes of breast cancer as determined by RSSPC using bc-GenExMiner database v3.0. A. The figure and table show the patients with low, intermediate and high MIEN1 expression in each molecular subtype. c MIEN1 protein expression was analyzed by immunoblotting analysis and β-actin was used as loading control. d Kaplan Meier survival curve showing the survival percentage of breast cancer patients were significantly low in MIEN1-high expressing tumors compared to low-expressing cohort. TCGA data analyzed using UCSD cancer genome browser