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Fig. 6 | Molecular Cancer

Fig. 6

From: MIEN1, a novel interactor of Annexin A2, promotes tumor cell migration by enhancing AnxA2 cell surface expression

Fig. 6

AnxA2 and MIEN1 silencing inhibit tPA dependent plasmin generation. Plasmin activity was determined at 460 nm using recombinant plasminogen, TPA and fluorogenic plasmin substrate D-VLK-AMC. Western blotting was performed to confirm depletion of MIEN1 and AnxA2 in HCC-70 (a) and MCF-7 (c) cells following siRNA knockdown. PGK served as a loading control. Total fold change in plasmin level in HCC-70 (b) and MCF-7 (d) cells was calculated by normalizing the initial rates of plasmin in untreated cells (which were assigned a value of 1). The data is presented as means ± s.d. (n = 6 for untreated controls and n = 6 for siRNA treatments)

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Molecular Cancer

ISSN: 1476-4598