Fig. 3
a. Surviving fraction of unsorted MDA-MB-231 cells and CSCs (CD44+/CD24-) and non-CSCs (CD44-/CD24-) delivered from MDA-MB-231 cells plated immediately after carbon ion beam or X-ray irradiation. The graphs show the mean and standard error calculated from three independent experiments. All experiments were performed in triplicate (n = 3). b. Quantification of colony formation of (CD44+/CD24-) and non-CSCs (CD44-/CD24-) after X-ray, a carbon ion beam alone or in combination with 25 μM of CDDP. CDDP was added prior to irradiation and treated for 5 days. *, p < 0.01, compared to non-CSCs. Representative photos and quantification of spheroid size formed from MDA-MB-231 delivered CSCs (CD44+/CD24-) (c) and MDA-MB-453 delivered CSCs (ESA+/CD24-) (d) after X-ray, a carbon ion beam alone or in combination with 25 μM of CDDP. The spheroid formation was observed 7 days after X-ray, a carbon ion beam alone or in combination with CDDP. CDDP was added prior to irradiation and treated for 5 days. The graphs show the mean and standard error calculated from three independent experiments. *, p < 0.01, compared to non-CSCs. All experiments were performed in triplicate (n = 3)