Fig. 6
a. Quantification and representative photos of nuclear γH2AX foci formation in CSCs (CD44+/CD24-) delivered from MDA-MB-231 cells at 1 h and 24 h after a carbon ion beam, X-ray alone or in combination with 25 μM of cisplatin (CDDP). CDDP was added prior to irradiation and treated for 24 h. Data represent mean ± SD. *p < 0.05 compared to non-CSCs. b. Quantification and representative photos of nuclear γH2AX foci larger than 1.5 μm2 after 24 h carbon ion beam combined with 25 μM of CDDP in CSCs (CD44+/CD24-) delivered from MDA-MB-231 cells. Data represent mean ± SD. *p < 0.01 compared to CDDP alone treated cells. c. Quantification and representative photos of nuclear γH2AX foci formation in CSCs (ESA+/CD24-) delivered from MDA-MB-453 cells at 24 h after a carbon ion beam, X-ray alone or in combination with 25 μM of CDDP. CDDP was added prior to irradiation and treated for 24 h. Data represent mean ± SD. *p < 0.05 compared to non-CSCs. Arrows indicate γH2AX foci larger than 1.5 μm2. *p < 0.01 compared to γH2AX foci sizes in X-ray irradiated cells. All experiments were performed in triplicate (n = 3)