Fig. 2
Knockdown of Na,K-ATPase β1-subunit in medulloblastoma cells increases cell proliferation. a. Na,K-ATPase β1-subunit protein levels in two independent clones of shRNA-mediated β1-subunit knockdown in DAOY cells (Sh-NaKβ-cl1 and cl2). ShV-cl1 and cl2 are respective scrambled shRNA transfected control clones that were obtained in parallel with each of the two independent selections. b. Quantification of β1-subunit levels in knockdown cells relative to the levels in the respective control clones from three (Sh-NaKβ-cl1 and ShV-cl1; p < 0.005) or two (Sh-NaKβ-cl2 and ShV-cl2) immunoblots. c. Na,K-ATPase β2-isoform and α1-subunit expression in β1-subunit knockdown cells. d. Cell proliferation of β1-subunit knockdown clones expressed as fold change relative to cell number at day 0. The differences in fold change between ShV-cl 1 and Sh-NaKβ-cl 1 were statistically significant at days 2, 3, and 4 (p < 0.05). e. BrdU uptake in β1-subunit knockdown cells. There was a significant difference in percent BrdU positive cells between cell lines (p < 0.005, two way ANOVA). The difference in percent BrdU positive cells was significant starting on day 3 (* = p < 0.05)