Fig. 3
Tumor xenografts of Na,K-ATPase β1-subunit knockdown cells. a. Tumor volume of subcutaneous xenograft tumors injected into immunocompromised mice (n = 12). The difference in the overall mean volume between the group of ShV-cl1 and Sh-NaKβ-cl1 tumors was significant (p < 0.05, determined using a mixed effect model with AR(1) correlation structure). b. Kaplan-Meier survival curve of mice with ShV-cl1 (ShV) and Sh-NaKβ-cl1 (Sh-NaK-beta) tumors (p < 0.05). c. Average β1-subunit mRNA levels from three representative tumors of ShV-cl1 and Sh-NaKβ-cl1 cells normalized to beta-2 microglobulin. The difference in mRNA expression was significant (** = p < 0.005). d. β1-subunit protein levels in xenograft tumors shown in c. An immunoblot for tubulin served as loading control. e. Cyclin D1 levels in xenograft tumors shown in c. An immunoblot for tubulin served as loading control