Fig. 5
Inverse relationship between Bmi1 and Na,K-ATPase β1-subunit levels in medulloblastoma and CGP cells. a. Protein extracts obtained from four Smo/Smo medulloblastoma and cerebellum of four wild type mice were immunoblotted with the indicated antibodies. α-tubulin was used as loading control. b. mRNA levels of Bmi1 and β1-subunit in medulloblastoma and cerebellum as determined by qRT-PCR. c. Protein levels of Bmi1 in differentiating CGP cells. GAPDH was used to ensure equal loading. d. mRNA levels of Bmi1 and β1-subunit in CGP cells differentiating in vitro. The difference in β1-subunit mRNA expression was significant during CGP differentiation (** = p < 0.001, one way ANOVA), while the difference in Bmi1 expression was not significant (one way ANOVA). e. CGP cells grown in vitro were exposed to either DMSO or 0.1 μM SAG for indicated time points and the lysates were immunoblotted with the indicated antibodies. GAPDH served as loading control