Skip to main content
Fig. 1 | Molecular Cancer

Fig. 1

From: Long non-coding RNA TUG1 is up-regulated in hepatocellular carcinoma and promotes cell growth and apoptosis by epigenetically silencing of KLF2

Fig. 1

Relative TUG1 expression in HCC tissues and HCC cell lines and TUG1 was regulated by SP1. a Relative TUG1 expression in HCC tissues (n = 77) compared with corresponding non-tumor tissues (n = 77). TUG1 expression was examined by qPCR and normalized to GAPDH expression. Results were presented as ΔCT in tumor tissues relative to normal tissues. b Relative TUG1 expression levels of HCC cell lines (HepG2, Hep3B, MHCC-97H) compared with that in the normal hepatic epithelium cell line (L02). c ChIP–qPCR of SP1 occupancy and binding in the TUG1 promoter in HepG2 and Hep3B cells, and IgG as a negative control. d Luciferase reporter assay was performed by co-transfecting the TUG1 promoter fragment (pGL3-TUG1) or deleted TUG1 promoter fragment with SP1 expression vector or an empty vector in HepG2 cells. e The SP1 expression level was determined by qPCR when HepG2 cells transfected with si-SP1. f The TUG1 expression level was determined by qPCR when HepG2 cells transfected with si-SP1. g The SP1 expression level was determined by qPCR when Hep3B cells transfected with si-SP1. h The TUG1 expression level was determined by qPCR when Hep3B cells transfected with si-SP1. i The SP1 expression level was determined by qPCR when HepG2 cells transfected with EGFP-SP1. j The TUG1 expression level was determined by qPCR when HepG2 cells transfected with EGFP-SP1. k The SP1 expression level was determined by qPCR when Hep3B cells transfected with EGFP-SP1. l The TUG1 expression level was determined by qPCR when Hep3B cells transfected with EGFP-SP1. m,n The SP1 protein expression level was determined by Western blotting when HepG2/Hep3B cells transfected with si-SP1. o,p The SP1 protein expression level was determined by Western blotting when HepG2/Hep3B cells transfected with EGFP-SP1

Back to article page