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Fig. 6 | Molecular Cancer

Fig. 6

From: Shikonin-enhanced cell immunogenicity of tumor vaccine is mediated by the differential effects of DAMP components

Fig. 6

Cytokine profiling array analysis of conditioned culture media from various 4 T1 TCL-treated DCs. a Cytokine array membranes were incubated with cultured media from immature DCs or DCs that were treated with different TCL samples, including Naive-TCL, Dox-TCL, SK-TCL, SK-TCL(−Hsp70) , SK-TCL(−CRT) and SK-TCL(−HMGB1) for 2 h, and then stimulated with LPS for another 22 h. Squares mark the chemokines secreted from DCs that were increased in the SK-TCL-loaded group, as compared with those from Naive-TCL-loaded groups. Green square: G-CSF, blue square: SDF-1/CXCL12, red square: MIP-1α/CCL3 and yellow square: MCP-1/CCL2. b Stimulation (in fold change) of ICD-responsive chemokines in DCs loaded with Naive-TCL, Dox-TCL, SK-TCL, SK-TCL(−Hsp70), SK-TCL(−CRT) or SK-TCL(−HMGB1). Secretion level of each chemokine was normalized by value of Naive-TCL-loaded group (Fold change = Each TCL-loaded group/Naive-TCL-loaded group). Color of cone for each chemokine is correspondent to the indication in (a)

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