Fig. 4

ALDOB inhibits HCC metastasisin vivo. a Images of lung metastases that developed in Huh7-pgl4.17 lateral tail vein injection models by the IVIS Imaging System. Representative luciferase signals captured in each group at the initial injection and 48 days after the injection of the cells are shown. The statistical analysis is shown in (b). c Representative H&E stained images of lung metastatic loci from each group in A. The statistical analysis is shown in (d). e Images of lung metastases that developed in SMMC7721 lateral tail vein injection models by the IVIS Imaging System. Representative GFP signals were captured in each group 49 days after the mice were euthanized. The statistical analysis is shown in (f). g Representative image of intrahepatic metastases 8 weeks after orthotopic xenograft transplantation of the indicated SMMC7721 cell clones in nude mice. The number of intrahepatic metastases in each group is shown in (h). i The relative levels of human LINE1 DNA were analyzed by qPCR of genomic DNA from whole-blood samples 48 days after orthotopic xenograft transplantation of the indicated SMMC-7721 cell clones; expression levels were normalized to mouse LINE1 DNA. jThe number of GFP-labeled CTCs was examined by flow cytometry from whole-blood samples. The statistical analysis is shown in (k)